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The quality of the raw plants and the prepared diets will be controlled by analyzing nutritional (proximates, micronutrients) and deteriorating ingredients (mycotoxins, pesticides, metals), microbiological quality, and intended and unintended GMO which may affect the trial outcomes. Lists of essential analytes have already been identified within GRACE (see the GRACE study plan). Analyses shall be carried out in certified laboratories.

 

Transcriptome, proteome and metabolome analyses of the GM crop plant materials and conventional counterparts will be performed. The aim is to apply omics strategies to detect unintended effects in the GM crop plant materials. For the different types of omics studies (transcriptomics, proteomics and metabolomics) well-established protocols are available and will be applied, similar to those adapted to maize grain currently used in the frame of the GRACE project. The same materials that have been used to prepare the animal feed will be analyzed.

 

Data collation and bioinformatics analysis

The data obtained from the analysis of plant material will be further analyzed by making use of well-established bioinformatic tools. An additional in-silico analysis will be applied to identify the genes, proteins or metabolites that have altered levels in the physiology of the GM plant versus the conventional counterpart and to further elucidate pos sible alterations in the plant physiology.
The results of omics studies will be correlated with the outcome of the compositional analysis of the respective GM and conventional plant materials.

 

The omics data will also be compared with those of other studies that were performed to identify unintended effects of the genetic modifications e.g. available in the literature or through other projects such as GRACE. The database CADIMA will compile the results obtained in safety assessment studies. G-TwYST will be able to integrate and reconsider outputs from GRACE and collate potential toxicity of the alterations found in the GM crop plants vs. conventional counterparts. Inclusion of this new set of data will increase the potency of the database, so that it can be used to assess new GMOs in the future.


 

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G-TwYST results published

13 February 2019

This week the paper  "Lack of adverse effects in subchronic and chronic toxicity/carcinogenicity studies on the glyphosate-resistant genetically modified maize NK603 in Wistar Han RCC rats" has been published in Archives of Toxicology. The open source paper is available online at https://doi.org/10.1007/s00204-019-02400-1.

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G-TwYST data available at CADIMA

13 February 2019

G-TwYST has  made accessible the detailed scientific information including raw data via the open access database CADIMA set up by the FP7 project GRACE.

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